Spectrofluorimeter

 Provides ultimate analytical speed and sensitivity in fluorescence research. Fluorescence spectroscopy plays a major role in the analysis because it provides high sensitivity and high specificity for functional compounds. High sensitivity is due to the wavelength difference between the excited radiation and the fluorescence.

 These results lead to a signal opposite to the background of essentially zero. The above characteristic is due to the dependence on two spectra: the excitation spectrum and the diffusion spectrum. 3D matrix scanning and contour mapping provide a unique fingerprint that qualitatively identifies a composition. In fluorescence analysis, the amount of light irradiation specifically under appropriate stimulation is used as a measure of the concentration of responsible species.

Specifications of spectrofluorimeter device

Stimulation: 200-950 nm range

Emission: 200-950 nm range

Bandpass: 0-15 nm, continuously adjustable via PC

Merge time: from 1 ms to 160 s

Scanning speed: 80 nm

Raman water signal: at least 400,000 cpm at 350 nm excitation, 397 nm emission, 5 nm pass band, 1 second integration time

Signal / noise ratio: 3000/1

Single thermostat cuvette holder. Designed for one meter length of Covet route. Maintains temperature from -20 to 80 ° C

Spectrofluorimeter applications

Estimation of nuances in the tertiary structure of proteins by monitoring the intrinsic tryptophan fluorescence of proteins.

Determination of binding constants of small molecules (such as polyphenols) to proteins by tryptophan fluorescence quenching method

Investigation of protein surface hydrophobicity (or small changes in tertiary structure that affect surface hydrophobicity) by monitoring the binding of hydrophobic fluorescent probes (such as ANS, 8-anilino-1-naphthalenesulfonic acid).

Millard reaction monitoring by detecting Millard fluorescent reaction products.

Highly sensitive determination of enzyme activity with fluorescent substrate.

Evaluation of the interactions of biologically active compounds, such as platinum (IV) and ruthenium (II) complexes, with human serum albumin and calf thymus DNA.

Correlation of structure and properties of natural and synthetic molecules and their metal complexes

Source: https://www.chem.bg.ac.rs/cmnh/oprema/fluoromax_4-en.html